In Vitro Gliding Assays Indicate that Chlamydomonas Dynein Moves Microtubules Polymerized from Chlamydomonas Axonemal Tubulin Faster than those Polymerized from Porcine Brain Tubulin
نویسندگان
چکیده
منابع مشابه
Displacement-weighted velocity analysis of gliding assays reveals that Chlamydomonas axonemal dynein preferentially moves conspecific microtubules.
In vitro gliding assays, in which microtubules are observed to glide over surfaces coated with motor proteins, are important tools for studying the biophysics of motility. Gliding assays with axonemal dyneins have the unusual feature that the microtubules exhibit large variations in gliding speed despite measures taken to eliminate unsteadiness. Because axonemal dynein gliding assays are usuall...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2012
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2011.11.2030